Journal: iScience
Article Title: Multi-omics analysis reveals features improving outcomes and immunotherapy resistance in MSS colorectal cancer
doi: 10.1016/j.isci.2026.114817
Figure Lengend Snippet: CADM1 enhances tumor cell stemness to promote proliferation, invasion, and metastasis of CRC (A) The flowchart of MLDMPIS malignancy-associated gene screening. (B and C) The significantly positively (B) and negatively (C) correlated GSEA functional pathways with MLDMPIS. (D) Venn diagram of the intersection between function-driver and modeling genes of MLDMPIS. (E and F) Kaplan-Meier survival analysis between high and low CADM1 expression groups in the TCGA-CRC (E) and meta cohorts (F). (G) Differential expression of CADM1 in MSI-H and MSS CRC patients. (H and I) Correlation analysis between CADM1 expression and tumor metastasis status in GSE64256 (H) and GSE41258 (I) cohorts. (J) The significantly positively correlated GSEA functional pathways with CADM1 expression. (K and L) Validation of CADM1 gene knockdown efficiency in SW480 (K) and CT26 (L) CRC cell lines (data are presented as mean ± SD from n = 5 independent experiments). (M and N) Results of CCK-8 cell proliferation activity assay between CADM1 knockdown and control groups in SW480 (M) and CT26 (N) CRC cell lines (data are presented as mean ± SD from n = 5 independent experiments). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
Article Snippet: CCK-8 Cell Proliferation and Cytotoxicity Assay Kit , Solarbio , Cat #CA1210.
Techniques: Functional Assay, Expressing, Quantitative Proteomics, Biomarker Discovery, Knockdown, CCK-8 Assay, Activity Assay, Control